- A “shomer shabbos Jew” (a man over the age of Bar Mitzvah or a woman over the age of Bat Mitzvah who is properly observant of Jewish law) should separate Challah.
- A non-Jewish person cannot separate Challah. If he or she does so, it is invalid.
- In circumstances, outside of Israel (where the separation of Challah is a Rabbinic ordinance) where an observant Jew is not present to take Challah, a non-Jewish baker or worker may take a handful of dough from each of the main batches of dough and put it aside for an observant Jewish person to later take Challah from the pieces that were put aside.